I work at the University of Toronto under the supervision of Prof. Lewis Kay. My current focus is on understanding the molecular and biophysical basis for formation of membraneless organelles. Membraneless organelles are a relatively newly discovered class of intracellular organelles that are thought to arise from phase separation of protein and RNA components to form functional compartments for cellular processes to occur.
During my PhD I studied a protein called Yop1p, which is responsible for generating the high membrane curvature found in the tubular endoplasmic reticulum (ER). Follow this link if you'd like to learn more...
Brady JP, Farber PJ, Sekhar A, Lin YH, Huang R, Bah A, Nott TJ, Chan HS, Baldwin AJ, Forman-Kay JD, Kay LE. Structural and hydrodynamic properties of an intrinsically disordered region of a germ cell-specific protein on phase separation. Proc Natl Acad Sci U S A. 2017 Sep 26;114(39):E8194-E8203. doi: 10.1073/pnas.1706197114. Epub 2017 Sep 11.
Huang R, Brady JP, Sekhar A, Yuwen T, Kay LE. An enhanced sensitivity methyl 1H triple-quantum pulse scheme for measuring diffusion constants of macromolecules. J Biomol NMR. 2017 Jul 17. doi: 10.1007/s10858-017-0122-9.
Breeze E, Dzimitrowicz N, Kriechbaumer V, Brooks R, Botchway SW, Brady JP, Hawes C, Dixon AM, Schnell JR, Fricker MD, Frigerio L. A C-terminal amphipathic helix is necessary for the in vivo tubule-shaping function of a plant reticulon. Proc Natl Acad Sci U S A. 2016 Sep 27;113(39):10902-7. doi: 10.1073/pnas.1605434113. Epub 2016 Sep 12.
Brady JP, Claridge JK, Smith PG, Schnell JR. A conserved amphipathic helix is required for membrane tubule formation by Yop1p. Proc Natl Acad Sci U S A. 2015 Feb 17;112(7):E639-48. doi: 10.1073/pnas.1415882112. Epub 2015 Feb 2.
Cehovin A, Simpson PJ, McDowell MA, Brown DR, Noschese R, Pallett M, Brady J, Baldwin GS, Lea SM, Matthews SJ, Pelicic V. Specific DNA recognition mediated by a type IV pilin. Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):3065-70. doi: 10.1073/pnas.1218832110. Epub 2013 Feb 5.
Graham SA, Jegouzo SA, Yan S, Powlesland AS, Brady JP, Taylor ME, Drickamer K. Prolectin, a glycan-binding receptor on dividing B cells in germinal centers. J Biol Chem. 2009 Jul 3;284(27):18537-44. doi: 10.1074/jbc.M109.012807. Epub 2009 May 6.
Yuwen T, Bah A, Brady JP, Ferrage F, Bouvignies G, Kay LE. Measuring Solvent Hydrogen Exchange Rates by Multifrequency Excitation (15)N CEST: Application to Protein Phase Separation. J Phys Chem B. 2018 Sep 19. doi: 10.1021/acs.jpcb.8b06820.
Yuwen T, Brady JP, Kay LE. Probing Conformational Exchange in Weakly Interacting, Slowly Exchanging Protein Systems via Off-Resonance R1rho Experiments: Application to Studies of Protein Phase Separation. J Am Chem Soc. 2018 Feb 14;140(6):2115-2126. doi: 10.1021/jacs.7b09576. Epub 2018 Jan 31.
Here are some apps that I wrote in Python for analysis of protein sequences and NMR relaxation data.
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